Human milk lipases. I. Serum-stimulated lipase
نویسندگان
چکیده
منابع مشابه
Two forms of human milk bile-salt-stimulated lipase.
This study provides the first direct evidence for the existence of two active forms of the human milk enzyme bile-salt-stimulated lipase. Heparin-based affinity chromatography and size-exclusion chromatography were used to obtain two active forms of this enzyme with molecular masses of 97 and 120 kDa.
متن کاملActivation of human milk lipase.
The presence of a serum-activated lipase (lipoprotein lipase) in bovine milk was first reported by Quigley et al. (1958). A similar lipolytic system has been studied in milk of various species where it is assumed to be the predominant lipid-hydrolysing enzyme (Korn, 1962). It was found to be unable to hydrolyse milk triglycerides; its presence in milk has been explained as a result of a penetra...
متن کاملSequence identity between human pancreatic cholesterol esterase and bile salt-stimulated milk lipase.
Three overlapping cDNA clones covering the entire primary sequence of the bile salt stimulated lipase in human milk were isolated from a human breast lambda gt10 cDNA library by screening with the rat pancreatic cholesterol esterase cDNA. Nucleotide sequencing of the cDNA showed that the human milk lipase mRNA encodes a 748-residue protein, including a 23-residue signal peptide. The human milk ...
متن کاملHuman Milk Bile Salt-activated Lipase
Further studies on human milk bile salt-activated lipase were performed toprovide kinetic and additional chemical characterizations of this enzyme. The enzyme was homogeneous by urea-sodium dodecyl sulfatepolyacrylamide gel electrophoresis and isoelectric focusing with an isoelectric point of 3.7. A unique feature of the amino acid composition of this enzyme was a high proline content (13 mol %...
متن کاملThe complete digestion of human milk triacylglycerol in vitro requires gastric lipase, pancreatic colipase-dependent lipase, and bile salt-stimulated lipase.
Gastric lipase, pancreatic colipase-dependent lipase, and bile salt-stimulated lipase all have potential roles in digestion of human milk triacylglycerol. To reveal the function of each lipase, an in vitro study was carried out with purified lipases and cofactors, and with human milk as substrate. Conditions were chosen to resemble those of the physiologic environment in the gastrointestinal tr...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1974
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)36784-5